Bull breeding soundness examination (BBSE)

John Bertram
Formerly Queensland Government

Given that a beef business is essentially driven by the number of animals it can sell, their weight and price, then there is a need to focus on the relative areas that will drive that profit. Research had shown fertility is often five to ten times more important than price received in terms of enterprise profitability. Optimizing fertility performance is obviously a big issue for all northern beef herds.

Producers can often spend a lot of effort in selection of replacement females, and frequently become so focused on the environment of their production system, they fail to place the necessary emphasis on genetic selection of herd sires. The herd sires set the direction of profitability for many years to come. Selection of sires based on appearance alone has limited has limited the beef industry’s rate of gain. Only about 10–15% of the 30,000 plus replacement bulls needed to fill requirements in north Australia each year are currently sold with any fertility measurement describing their function. This has to improve if the industry is going to continue to make significant genetic progress.

Australian Association of Cattle Veterinarians have determined that fertility is the ability of a bull to achieve, by natural service, a pregnancy rate of 60% and 90% in 50 normally cycling females, within three and nine weeks of mating, respectively. The beef industry is focused on profitability producing a calf from each breeder within a 365 day interval. The stud industry must have the same focus if they wish to remain viable and relevant.

How can fertility be improved?

Objective information in the form of a Bull Breeding Soundness Evaluation (BBSE) in terms of “what we see” (phenotype) is essential and BREEDPLAN EBVs are necessary from a genetic perspective.

The BBSE was developed by veterinarians to standardize bull fertility testing and to provide a consistent descriptor of bull fertility. The evaluation indicates whether a bull has met a set of standards for key fertility components which indicate whether a bull has a high probability of being fertile. The components are:

  • Scrotal circumference (cm) and tone or resilience
  • Physical examination for faults in the head, legs, joints, feet, sheath and penis
  • Semen analysis for motility,
  • Morphology (or structure of the individual sperm cells), and
  • Mating behaviour / mating ability.

The Australian Association of Cattle Veterinarians (ACV) oversees the BBSE practices with appropriate certification. The certificate provides a standard of assessment for veterinary evaluation of the various reproductive traits important to beef producers. This evaluation is conducted prior to sale and details the identification of the sire, date and location where the evaluation was conducted, the assessments made and relevant disease information (as shown in the example in Figure 2). It is purely an evaluation of a range of measures on that date on which it was done and does not provide any guarantee or imply the number of calves that the bull will sire in either single or multiple sire matings.

The BBSE is not a genetic evaluation of reproductive traits, but an indication of the animal’s present reproductive function. However, this certificate is far superior to ‘the lack of’ or ‘distorted’ information that is frequently available to many bull buyers. This evaluation can also be conducted on property as an annual bull test prior to mating to identify any bull that is declining in fertility.

In the sale catalogues, bull sellers are able to provide in association with each bull in the catalogue, or list, a summary of the tests conducted by the examining veterinarian. This BBSE summary (Figure 1) will be in the form of:

Bull
Number/name
Age
Yr:Mn
Scrotum Physical Semen Morphology Serving
AACV Top of the Rack 2.02 37.0cm P nt

Key

37.0 = Scrotal size or circumference in centimetres
a = Pass
P = OK under natural mating
r = Fail
na = Not applicable
nt = Not tested.

A summary of the five components of bull fertility in the BBSE follows:

Scrotum: Scrotal circumference/size (SS) in centimetres (cm) where testes shape is within normal range. The current recommendations for tropically adapted bulls are a minimum scrotal size of 32cm (and average is 34–36cm) for a two-year-old bull.

Physical: Within the constraints of a standard examination, there is no evidence of any general physical/structural condition or of a physical condition of the reproductive tract indicating sub-fertility or infertility. This evaluation will identify structurally unsound bulls in legs, feet, sheath and general structure.

Semen: Crush-side assessment indicates that the semen is within normal range for motility, colour and percent progressively motile and is suitable for laboratory evaluation.

Morphology: Semen examination of percent normal sperm using high power magnification to ensure minimum standards for normal function are achieved.

Serving: The bull is able to serve normally as demonstrated in a standard test and shows no evidence of fertility limiting defects.

The resultant certificate (see Figure 2) should be sought out by bull buyers when choosing between bulls as they also provide details of aspects of the evaluation that cannot be seen in the live animal e.g. percent normal spermatozoa and mating behavior details (if conducted).

Many of us are very comfortable in discussing the feet, leg and joint structures in animals and are very confident in making a selection decision to purchase or otherwise or use a bull based on what we see. The features of a bull that are clearly visible need little reinforcing in the selection process. However, what we can not see (without a microscope) often needs a little explanation.

Understanding the morphology and why it is important

Morphology in a BBSE is basically the ‘structure’ of individual sperm cells. The structural attributes that are not clearly visible (or that require a microscope to view) are frequently just as important, if not more important as far as affecting a bulls fertility.

Unlike the female that has her ‘quota’ of eggs at the start of life, the bull is continuously producing semen within the tubules in the testicles. The testicles are about 2°C cooler than body temperature. Between the head, body and tail of the epididymis there is a long tube for storage and maturation of the spermatozoa produced. This production pipeline takes about 6–8 weeks from the start of production to when the semen is ready for ejaculation. As semen is continually produced, unused semen is excreted in the urine of all bulls.

So can a bull’s fertility change?

How often do we think about what affect various treatments we apply to a bull, have on his fertility? For example, when a bull is bought at a sale after being ‘stuffed full of feed’ (or more nicely put ‘prepared for sale’) and we then ‘let him down’ (reduce his weight considerably); do we affect his fertility? When a new bull has a fight with the current herd sires and is lame for a few days; does that affect his fertility? In fact any stress that we place on a bull, either as part of our production system or our husbandry program, can affect the quality or structure of the spermatozoa in the continuous production of semen from within the testicles to each of the storage glands along the reproductive tract.

Can we examine and categorize the semen produced by a bull?

Yes. Many breeders will be aware that upon the collection of semen either by electro-ejaculation or rectal massage, semen is examined crush-side for colour (no blood or urine staining allowed) and density of spermatozoa which is ranked on a 1 (clear to cloudy) to 5 (thick creamy colour) basis. In addition, when using low power magnification, the amount of swirl or vigorous swimming motion of all the spermatozoa is scored on a 1 (no swirl; generalized flickering of individual sperm only) to 5 (fast distinct swirl with continuous dark waves) basis. Once completed, the percentage of individual sperm that are swimming forward freely and independently is recorded. This assessment is a compulsory measure of fertility to meet BBSE standards. Based on extensive research the ACV has determined that a threshold of 30% progressively motile sperm is a pass on a BBSE. In addition, the sample must be free of large numbers of pus cells.

The final evaluation of the semen is the percentage of individual spermatozoa that are structurally normal – the morphology. To record the % Normal sperm, a sample of the semen collection is placed in a small tube with a special diluent and sent off to one of the accredited morphologists. The morphologist will examine 100 individual spermatozoa and record all the abnormalities present.

The morphologist report will detail the % Normal sperm and the percentages of abnormalities. The % Normal threshold indicated by the ACV is >70% Normal for bulls used in single sire mating or for Artifical Breeding (AI), and >50% normal for bulls used in multiple sire mating. However some producers may choose to use bulls with higher than 50% Normal. This trait is heritable, and is genetically correlated to the interval from calving to first oestrus cycle after calving in females (i.e. in the daughters).

Percentages are detailed for the following abnormalities: % Proximal Cytoplasmic Droplets (PD); % Mid-Piece Abnormalities (MP); % Abnormal Tails and Loose Heads (T&H); % Pyriform Heads (Py); % Knobbed Acrosomes (KA); % Vacuoles & Teratoids (V&T); % Swollen Acrosomes (SA). Maximum thresholds have been set for each of these categories based on their potential impact on the fertility of the bull e.g. PD- 20%, MP- 30%, T&H- 30%, Py- 20%, KA-30%, V&T- 20% and SA- 30%.

A frequent concern by producers is – why do subsequent samples from the same bull differ in % Normal sperm?

As outlined previously, the spermatozoa are continuously produced and are therefore subject to any stressors in the body (including rise in body temperature, disease, etc.) until the time of ejaculation or excretion. Because the stress or rise in body temperature may have been over an extended time, it will have differing effects on the spermatozoa depending on where they were at, in the production process, on the occasion of the stress / temperature. The morphology result is an indication of the quality of the semen at that point in time alone. However, if the % Normal is high, the bull has somewhat more of a buffer and can afford to decrease more in % Normal as a result of stress than a bull that inherently / genetically produces low % Normal semen. If on the first sample, a bull produces a low % Normal sperm, then the bull should be retested between 42 and 56 days after the first test to gain a more representative sample of his true semen quality. That of course is assuming that the bull is not suffering from any longer term adverse affects or ongoing stress.

Therefore, when a BBSE is conducted and the morphology results are presented, there should always be an interpretation of the results in light of the bull’s vaccination history, any prior stress imposed on the bull and the bull’s age and scrotal size. The semen sample tells us something about the bull’s fertility when the sample was taken. When associated with subsequent or previous samples, we can gain a better understanding of the potential fertility of the bull.

Are all the details in a BBSE always available?

Unfortunately NO. Veterinarians often only do the components of the BBSE that the stud breeder requests. Furthermore, whilst some breed societies have supported the BBSE process, in principal, they have NOT gone so far as requiring a COMPLETE BBSE as a prerequisite for bulls to be included in a breed society endorsed sale or multi-vendor sale. It is therefore, essential for all bull buyers to seek out and cite the certificate in advance of purchasing your “new investment” as it is too late after the bull has been assigned to you in the auction. Alternatively, buy your bulls from vendors that clearly and openly identify the BBSE standards of their bulls. Interestingly, some studs request this information themselves when buying a bulls/s, but fail to provide this service to their clients in turn. The best feedback you can give to your bull supplier is to only buy / pay for bulls with the essential information, rather than many kilograms of grain from a feeder!

Managing the new investment – the new sire!

Just because a bull has four legs, a head and a set of testicles, it does not necessarily mean he is permanently a functional bull. How he is handled, what stressors he has undergone, the level of nutrition available and other factors can all have a strong effect (either positive or negative) on the ultimate fertility and functionality of that bull, and his fertility is dynamic, meaning it can change due to these and other stressors / circumstances.

Relocation to a new environment, subjecting bull to new viral and bacterial challenges, fighting and other issues associated with establishing new social groups, could all have often unseen impacts on semen quality. These impacts then flowed through to fertility in the herd.

Where possible, incoming bulls should be kept with animals of similar age, and bulls coming from very different geographic and climatic environments, should be relocated much earlier than those animals bought from a similar environment to your herd. Even within genotypes, some bulls adapt to more stressful environments more readily than others -these being more likely to have their semen quality affected.

Summary

Selection of bulls that meet minimum fertility standards has been a difficulty to many beef producers for a long time. For some time limited reproductive information has been included in sale catalogues, because many seedstock producers only supply the information that buyers either ask for or are prepared to pay for.

Regardless of whether you have sourced bulls out of the paddock or the sale ring, the need for some objectivity in selection remains the same. It is strongly recommended that bull buyers request a complete BBSE when sourcing bulls. Remember the bull with unknown fertility (and genetic merit for fertility) is going to affect herd function and profitability well into the next decade.

Figure 2. An example of a BBSE certificate that you – the bull buyer- can demand at the time of purchase, could be as follows:

Bullville Veterinary Clinic

Address Veterinarians
Station Road,
PO Box 30, Indooroopilly, Queensland 4068
Phone: 07 33787944, Fax: 07 38783559, Mobile: 0427 478 239
Email: bullivets@email.com.au
Dr Maree Jones
Dr Tom Smith
Dr Bill Chappel
Report: Bull Breeding Soundness Evaluation

This report was compiled exclusively for the use of the person to whom it is addressed. No other person or corporation has any authority to make use of any or all of this report.

This report is valid only when signed by the evaluating veterinarian and the bull’s owner or agent.

Summary

To: Mr Jack Shepherd, Handsome Station, Quilpe Queensland 4000

Place of examination: Handsome Station
Date: 03 July 2010
Brand: XXXX on offside
Breed: Santa Gertrudis
Bull:                                            Age:
Number/name:                          Yr : Month
Charter Stowers 1965              1 : 08

Scrotum Physical Semen Morphology Serving
39.5

nt

 

I hereby certify that information included in this report is in full accordance with the standards for evaluation and reporting bull breeding soundness as published by the Australian Association of Cattle Veterinarians Veterinarian:Signature:———————
I hereby certify that there has been no medical or surgical intervention of congenital abnormalities of the listed bull(s), whether genetic or not, to enable the above-mentioned standards to be met Owner/Agent:Signature:———————

 

Data Recorded:

Physical Serving
Condition Score 3 Serves 3
Testes Tone 3 Penis Normal
Penis Normal MS function Normal
Feet Normal
Legs 3
Leg joints Normal
Gait Normal
Head Normal
Semen
Density 4
Mass Activity 3
Motility 80%
Morphology
Normal Sperm 79%
General comment:

Bull Breeding Soundness Evaluation (BBSE)

In this presentation Alan Laing, Senior Beef Extension Officer with FutureBeef, outlines Bull Breeding Soundess Evaluation (BBSE) as a process to identify the bulls that are most likely to achieve pregnancies in breeders. 6:16 minutes published 3 May 2012 by FutureBeefAu.